Technology Transfer and Scale-Up of a High-Density E. coli Fermentation from a 200 L Stainless Steel to a 1,000 L Single-Use Fermenter

While single-use systems are common in mammalian cell culture, their adoption in microbial fermentation has been slower due to the high demands of oxygen transfer and cooling. ABEC’s Custom Single Run (CSR®) technology overcomes these challenges, offering single-use fermenters with the scalability and performance of stainless-steel systems.

BIOVECTRA leveraged ABEC’s CSR technology to scale a high-cell density E. coli process from a 200 L stainless-steel fermenter to a 1,000 L single-use fermenter. This collaboration demonstrated how single-use fermenters can deliver the performance of stainless-steel systems while offering the flexibility needed for diverse manufacturing needs and ensuring right-first-time scale up.

By overcoming key historical challenges in microbial fermentation, this approach highlights the potential for single-use technology to enable efficient scale-up and streamline multiple product production in a GMP environment.

Download this white paper to learn how scalable single-use systems can support reliable process performance and manufacturing flexibility.